ELISA Kit for Estradiol (E2) Pan-species (General) Competition ELISA

17B-Estradiol; Oestradiol; Beta-Estradiol

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Estradiol (E2) Packages (Simulation)
  • ELISA Kit for Estradiol (E2) Packages (Simulation)
  • ELISA Kit for Estradiol (E2) Results demonstration
  • CEA461Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Estradiol (E2) and the recovery rates were calculated by comparing the measured value to the expected amount of Estradiol (E2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-92 88
EDTA plasma(n=5) 80-101 88
heparin plasma(n=5) 96-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estradiol (E2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estradiol (E2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estradiol (E2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-104% 83-102% 86-101% 80-95%
EDTA plasma(n=5) 81-96% 83-94% 80-95% 78-101%
heparin plasma(n=5) 86-101% 80-94% 80-101% 84-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Estradiol (E2)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Estradiol (E2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Estradiol (E2) and unlabeled Estradiol (E2) (Standards or samples) with the pre-coated antibody specific to Estradiol (E2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Estradiol (E2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Estradiol (E2) in the sample.

Citations

  • Antiosteoclastic Activity of Milk Thistle Extract after Ovariectomy to Suppress Estrogen Deficiency-Induced OsteoporosisPubMed: PMC3678416
  • Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356
  • Utilizacao de células-tronco adultas para tratamento de falência ovariana causada por quimioterapia em camundongasLume: Source
  • The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar ratsInformahealthcare: Source
  • Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female ratsIjppsjournal: Source
  • Cell Therapy for Chemically Induced Ovarian Failure in MicePubmed:Pmc4274854
  • Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspringPubmed:24126190
  • Phloretin promotes osteoclast apoptosis in murine macrophages and inhibits estrogen deficiency-induced osteoporosis in micePubmed:24932975
  • Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapyTurkishjournalofurology:Source
  • Elevated Serum Bisphenol A Level in Patients with Dilated CardiomyopathyPubMed: 25996886
  • In uteroPubMed: 25530038
  • Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in RatsPubMed: 26629940
  • Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis periodPubMed: 25782373
  • Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarctionPubMed: 25770118
  • Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in ratsPubMed: 26556892
  • Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifersPubmed:27051212
  • Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorderPubmed:26934364
  • Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t MicrobiotaPubmed:27093642
  • Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat OffspringPubmed:26962114
  • Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARαPubmed:27413362
  • Assessment of ADMA, estradiol, and progesterone in severe preeclampsiaPubmed:27152507
  • A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in DendritogenesisPubmed:26977770
  • The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilizationpubmed:27876896
  • Molecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
  • Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
  • Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania150659
  • Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight womenS1756464617301561
  • Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum.pubmed:27838061
  • Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats.pubmed:28376377
  • Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARαpubmed:27413362
  • Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspringpubmed:27803384
  • Exposure of pregnant mice to triclosan impairs placental development and nutrient transport.pubmed:28322267
  • Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in ratspubmed:29049608
  • Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reductionpubmed:28973666
  • Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndromepubmed:28673204
  • Rcan2 and estradiol independently regulate body weight in female micepubmed:28624805
  • Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania10.5897/AJEST2016.2234
  • The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic micepubmed:29058779
  • Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance?10.1071/ANv57n12Ab065
  • A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized RatsISSN 2249 – 622X
  • The biocompatibility studies of polymer dots on pregnant mice and fetusespubmed:29071192
  • Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathwaypubmed:29020999
  • Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspringpubmed:28687593
  • Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cellspubmed:28656272
  • FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control studySuppl_2:728.1.abstract
  • The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicityPubmed:30021657
  • Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island populationPubmed:29525625
  • Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons10.1093:toxsci
  • Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
  • Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrationsPubmed:29775925
  • Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat modelPubmed:29705515
  • Differential Effects of Brain Death on Rat Microcirculation and Intestinal Inflammation: Female MalePubmed:29737476
  • Postnatal separation prevents the development of prenatal stress‐induced anxiety in association with changes in oestrogen receptor and oxytocin immunoreactivity in …Pubmed:29205599
  • DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathwayPubmed:29321035
  • Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian …Pubmed:29674607
  • Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neuronsDoi: 10.1016/j.biopha.2018.08.135
  • Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
  • Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the …Pubmed: 30606243
  • Possible Metabolic Alterations among Autistic Male Children: Clinical and Biochemical ApproachesPubmed: 30600432
  • Establishment and evaluation of a novel mouse model of peri/postmenopausal depressionDoi: 10.1016/j.heliyon.2019.e01195
  • Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
  • iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine
  • G protein-coupled estrogen receptor activation improves contractile and diastolic functions in rat renal interlobular artery to protect against renal ischemia …Pubmed: 30784936
  • Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve
  • G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide …Pubmed: 31034039
  • Aqueous extract of lily bulb ameliorates menopause-like behavior in ovariectomized mice with novel brain-uterus mechanisms distinct from estrogen therapyPubmed: 31207575
  • Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in …Pubmed: 31311554
  • Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopausePubmed: 31377402
  • Total Polysaccharides of Lily Bulb Ameliorate Menopause-Like Behavior in Ovariectomized Mice: Multiple Mechanisms Distinct from Estrogen TherapyPubmed: 31428228
  • Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinasePubmed: 31722718
  • Effect of FSH on E2/GPR30-mediated mouse oocyte maturation in vitroPubmed: 31704004
  • Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signalingPubmed: 31809938
  • Blocking of Estradiol Receptors ERα, ERβ and GPER During Development, Differentially Alters Energy Metabolism in Male and Female RatsPubmed: 31805254
  • Gambir Extract (Uncaria Gambir) Decreases Inflammatory Response and Increases Gastric Mucosal Integrity in Wistar Rats-Model GastritisPubmed: 31949507
  • Estradiol treatment during perinatal development altersPubmed: 31966342
  • Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cellsPubmed: 32334352
  • Lepidiline A Improves the Balance of Endogenous Sex Hormones and Increases Fecundity by Targeting HSD17B1Pubmed: 32239630
  • Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 …Pubmed: 33131727
  • Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio)Pubmed: 33011509
  • Impaired LTD-like motor cortical plasticity in female patients with major depression disorderPubmed: 32791084
  • Protective effects of irbesartan and benazepril against vaginal vascular remodeling and fibrosis in female spontaneously hypertensive ratsPubmed: 32790534
  • MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistancePubmed: 32757043
  • Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal ModelPubmed: 32517356
  • Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at pubertyPubmed: 32478927
  • Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female micePubmed: 32407858
  • Effect of estrogen-active compounds on the expression of RACK1 and immunological implicationsPubmed: 32328699
  • Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting …Pubmed: 33005806
  • Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
  • Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS
  • The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice
  • Moosense pedometer activity and periestrual hormone profile in relation to oestrus in crossbred cattle
  • Dihydrotestosterone regulates oestrogen secretion, oestrogen receptor expression, and apoptosis in granulosa cells during antral follicle development33465420
  • Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis33422655
  • BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose33508368
  • Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice
  • Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency
  • The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese …34417839
  • Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice34730391
  • Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in …34733331
  • 17β-Estradiol promotes angiogenesis of stria vascular in cochlea of C57BL/6J mice34822791
  • Effects of Treadmill Exercise on Anxiety-like Behavior in Association with Changes in Estrogen Receptors ERα, ERβ and Oxytocin of C57BL/6J Female Mice34746914
  • Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome MicePubmed:35057459
  • TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model micePubmed:35177657
  • ERα, but not ERβ and GPER, Mediates Estradiol-Induced Secretion of TSH in Mouse PituitaryPubmed:35138554
  • G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and …
  • Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency

Recommend products