ELISA Kit for Serglycin (SRGN) 
PRG; PPG; PRG1; proteoglycan 1, secretory granule
Overview
Properties
- Product No.CEC869Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunity
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Serglycin (SRGN) and the recovery rates were calculated by comparing the measured value to the expected amount of Serglycin (SRGN) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-91 | 81 |
| EDTA plasma(n=5) | 82-91 | 87 |
| heparin plasma(n=5) | 87-96 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serglycin (SRGN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serglycin (SRGN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Serglycin (SRGN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-96% | 92-101% | 91-105% | 95-102% |
| EDTA plasma(n=5) | 94-102% | 90-97% | 99-105% | 78-91% |
| heparin plasma(n=5) | 92-101% | 79-94% | 98-105% | 88-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Serglycin (SRGN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Serglycin (SRGN) and unlabeled Serglycin (SRGN) (Standards or samples) with the pre-coated antibody specific to Serglycin (SRGN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Serglycin (SRGN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Serglycin (SRGN) in the sample.
Giveaways
Increment services
Citations
- The role of increased synovial fluid A disintegrin and metalloproteinase with thrombospondin motifs4 and serglycin levels in osteoarthritisPubmed: 30536194
- SRGN, a new identified shear-stress-responsive gene in endothelial cellsPubmed: 32712749
- Significance of serglycin and its binding partners in autocrine promotion of metastasis in esophageal cancer33456569
- Cancer©\associated fibroblasts secrete hypoxia©\induced serglycin to promote head and neck squamous cell carcinoma tumor cell growth in vitro and in vivo by ¡33651283
- Serglycin induces osteoclastogenesis and promotes tumor growth in giant cell tumor of bone34556636