ELISA Kit DIY Materials for Complement C3 Convertase (C3 Convertase) Homo sapiens (Human) Assay Kit DIY Materials

C4b2a; C3bBb, complement C.hivin.4.hivin2

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.78-50ng/mL
  • Applicable Sensitivity0.31ng/mL
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  • ELISA Kit DIY Materials for Complement C3 Convertase (C3 Convertase) Packages (Simulation)
  • ELISA Kit DIY Materials for Complement C3 Convertase (C3 Convertase) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Complement C3 Convertase (C3c).No significant cross-reactivity or interference between Complement C3 Convertase (C3c) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Circulation levels of acute phase proteins in patients with Takayasu arteritisPubMed: 20100644
  • Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitisPubmed:27235358
  • Leishmania donovani Inhibitor of Serine Peptidases 2 Mediated Inhibition of Lectin Pathway and Upregulation of C5aR Signaling Promote Parasite Survival …Pubmed:29434593

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