ELISA Kit DIY Materials for Acetylcholine (ACH)

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,433.00 US$ 2,388.00 US$ 4,298.00 US$ 8,358.00 US$ 14,328.00
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Overview

Properties

Product No.KSA912Ge11
Organism SpeciesPan-species (General) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
DownloadInstruction Manual
CategorySignal transduction Infection immunity Neuro science

Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable Sampleserum, plasma and other biological fluids
Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
Detectable Range12.35-1000pg/mL
Applicable Sensitivity5.29pg/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Acetylcholine (ACH). No significant cross-reactivity or interference between Acetylcholine (ACH) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Combined administration of D-galactose and aluminium induces Alzheimer-like lesions in brainPubMed: 21614097
Neuroprotective effects of simvastatin and Cilostazol in L-methionine-induced vascular dementia in ratspubmed:27544235
Effect of statins on sirtuin 1 and endothelial nitric oxide synthase expression in young patients with a history of premature myocardial infarctionPubmed:29664427
Carotid baroreceptor stimulation in obese rats affects white and brown adipose tissues differently in metabolic protectionPubmed: 31126973
Caffeic acid phenethyl ester counteracts doxorubicin-induced chemobrain in Sprague-Dawley rats: Emphasis on the modulation of oxidative stress and …Pubmed: 33011510
Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and …Pubmed: 32954574
Autonomic nervous system receptor-mediated regulation of mast cell degranulation modulates the inflammation after corneal epithelial abrasionPubmed:35421396
Aqueous Ajwa dates seeds extract improves memory impairment in type-2 diabetes mellitus rats by reducing blood glucose levels and enhancing brain …Pubmed:35531250
Study on the mechanisms of Suaeda rigida polysaccharides on the heart inhibition and skeletal muscle promotion in the frog