ELISA Kit DIY Materials for Complement Factor H (CFH) Rattus norvegicus (Rat) Assay Kit DIY Materials

CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000ng/mL
  • Applicable Sensitivity5.77ng/mL
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  • ELISA Kit DIY Materials for Complement Factor H (CFH) Packages (Simulation)
  • ELISA Kit DIY Materials for Complement Factor H (CFH) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Complement Factor H (CFH). No significant cross-reactivity or interference between Complement Factor H (CFH) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Atypical hemolytic uremic syndrome: Korean pediatric seriesPubMed: 25443527
  • Circulating matrix metalloproteinases and procollagen propeptides in inguinal herniaPubmed:29484522
  • Fluorescent silver nanoclusters as antibody label in a competitive immunoassay for the complement factor HPubmed: 31187271
  • Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2JPubmed: 32585848
  • The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H33788378
  • A Family Affair: Addressing the Challenges of Factor H and the Related Proteins33868311

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