ELISA Kit DIY Materials for Epinephrine (EPI) Pan-species (General) Assay Kit DIY Materials

Adrenaline

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range24.69-2000pg/mL
  • Applicable Sensitivity9.74pg/mL
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  • ELISA Kit DIY Materials for Epinephrine (EPI) Packages (Simulation)
  • ELISA Kit DIY Materials for Epinephrine (EPI) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Epinephrine (EPI). No significant cross-reactivity or interference between Epinephrine (EPI) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • METHODS AND REAGENTS FOR THE DIAGNOSIS, PREVENTION AND TREATMENT OF INSULIN RESISTANCEFPO:EO237136
  • Glibenclamide attenuates myocardial injury by lipopolysaccharides in streptozotocin-induced diabetic micePubmed:Pmc4147163
  • Study of baicalin on sympathoexcitation induced by myocardial ischemia via P2X3 receptor in superior cervical gangliaPubmed:25554221
  • Catha edulis chewing effects on treatment of paranoid schizophrenic patientsPubMed: 25926735
  • Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic PolyuriaPubmed:27166674
  • Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesitypubmed:27694818
  • Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trialPubmed:29512230
  • Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance
  • RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose HomeostasisPubmed: 32328034
  • Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axisPubmed: 33177714
  • Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair …
  • Anti-stress activity of some plants extracts of the North Caucasus flora
  • Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D33549845
  • O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress33651993
  • Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy …Pubmed:35189708

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